School of Medicine Student Conference
Satu lagi poster yang akan tampil di bulan Desember 2010 ini:
Up-regulation of NO Production Induced by Maillard Product Derivatives and Activities of Natural Extracts Compounds Inhibiting NO Production in Murine Neuronal Cells
Knowledge related to advanced glycation ends (AGE) has been growing quite rapidly in recent years, especially with regard to its important role in diabetes, Alzheimer, and other aging-related diseases (Gasser and Forbes 2008). Protein glycation naturally occurred in vivo during normal metabolism and had been particularly observed in platelet cells and neurons. Glycation in a strict terminology (Zhang et al. 2008) is a non-enzymatic reaction that undergoes Schiff-base dan Amadori product re-arrangements and latter on adducts with protein, amino acids, or fragmented peptides. Apart formed inside cells, AGE was also obtained from food as a form of Maillard reaction derivatives (Deo et al. 2009). AGE concentration is proportional to the length of time and temperature used in cooking process.
Inside cells, nitric oxide (NO) means intercellular communication and has been regarded as a marker on oxidative stress conditions as well as an early indicator of inflammatory events (Sastre et al. 2006; Domek-Łopacińska and Strosznajder 2010). Murine neuroblastoma cells, Neuro2A (N2A), are known to be able to produce NO via enzymatic synthesis, through independent pathway (nNOS). Inducible pathway (iNOS) has been rarely reported in Neuro2A cell line, but could increase extracellular NO concentration up to three folds upon bacterial lipo-polysaccharides (LPS) activation (Lindegren et al. 2003).
Apigenin, Naringenin, Diosmetin are purified natural extracts from plants that have potential to be developed as a health supplement. These compounds have been proven to reduce inflammatory activity through down-regulation of pro-inflammatory cytokines and NO on immune cells (macrophage) (Chandler et al. 2010).
The first objective of this research is to determine the production of NO in conditions which are activated by AGE produced from sugar (glucose) and chicken egg albumin (AGE-CEA) and bovine serum albumin (AGE-BSA). As a positive control, a combination of LPS and interferon-gamma (IFN-γ) is used and negative control is non-activated cells. The second objective is to measure the rate of decrease in NO production in Neuro2A cells which are activated as a result of addition of potentially inflammatory protective compounds namely apigenin, naringenin, and diosmetin.
A poster will be posted soon...